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A new stromata-producing Neotyphodium species symbiotic with clonal grass Calamagrostis epigeios (L.) Roth. grown in China

     ¹ Nanjing Agricultural University, Nanjing, Jiangsu
² Microbiology, Nanjing, Jiangsu, 210095, China
³ Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

We describe a new stromata producing Neotyphodium species symbiotic with clonal Calamagrostis epigeios (L.) Roth. Stromata on the grass 47.5-186 mm long, occurred frequently, but neither perithecium nor mature ascus was observed. Morphology of 22 fungal isolates obtained from symptomatic and asymptomatic tillers were identical to each other, and similar to those of epichloë endophytes. In phylogenetic analysis, all of selected 5 fungal isolates clustered into a significantly distinct clade based on sequences of β-tubulin gene (tubB) introns and translation elongation factor 1- gene (tefA) introns with bootstrap values of 99%, supporting erect of a new species. Concerning the production of extremely long stromata on the host plants and absence of sexual spores, we propose "Neotyphodium stromatolongus Y. Ji, L. Zhan et Z. Wang, sp. nov." for this new species.

Key words: Calamagrostis epigeios, host specificity, stromata, morphology, phylogenetic analysis, Neotyphodium stromatolongus