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Validation of the name Pneumocystis wakefieldiae

     Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0560

Scott P. Keely
James R. Stringer

     Department of Molecular Genetics, University of Cincinnati College of Medicine, Biochemistry and Microbiology, Cincinnati, Ohio 45267-0554

	ADDENDUM

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When the name Pneumocystis wakefieldiae was proposed by us (Cushion et al 2004), we designated as type cryopreserved samples deposited at both the American Type Culture Collection (ATCC) and the Cincinnati Veterans Affairs Medical Center, without indicating which was the holotype. Unfortunately this contravention of Art. 37.6 of the International Code of Botanical Nomenclature (Greuter et al 2000) invalidated the name as a botanical (i.e. fungal) name. In addition, Art. 37.3 could be invoked because of some ambiguity in the number of isolates resulting from the pending ATCC number. We explicitly designate the holotype and location of deposit to validate the name.

Pneumocystis wakefieldiae sp. nov. (see Mycologia 96:433–434 2004 for Latin description) Holotypus: ATCC #PRA-87 (cryopreserved). Extracted on 23 Jan 2002 from the lungs of a Long Evans rat (Rattus norvegicus), which was infected naturally in a rat colony at the Cincinnati Veterans Affairs Veterinary Medical Unit, Cincinnati, Ohio, that harbored this Pneumocystis species and immunosuppressed 12 wk to provoke fulminate infection. This colony was established with rats from Charles River Laboratories, Inc., Wilmington, Massachusetts, in 1994. Isotypus: deposited in Cincinnati Veterans Affairs Medical Center, Medical Research Service as cryopreserved organisms in the Pneumocystis collection.

	ACKNOWLEDGMENTS

 
We thank Scott Redhead for alerting us to this problem and providing a solution.

	FOOTNOTES

 
Accepted for publication November 30, 2004.

¹ Corresponding author. E-mail: Melanie.Cushion{at}med.va.gov

	LITERATURE CITED

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Cushion MT, Keeley SP, Stringer JR. 2004. Molecular and phenotypic description of Pneumocystis wakefieldiae sp. nov., a new species in rats. Mycologia 96:429–438.[Abstract/Free Full Text]

Greuter W, McNeill J, Barrie FR, Burdet HM, Demoulin V, Filgueiras TS, Nicolson DH, Silva PC, Skog JE, Trehane P, Turland NJ, Hawksworth DL, eds. 2000. International Code of Botanical Nomenclature (Saint Louis Code). Adopted by the 16th International Botanical Congress St. Louis, Missouri, July–August 1999. Regnum veg. 138. Königstein, Germany: Koeltz Scientific Books. xviii + 474 p.

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