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Genetic variability of Phytophthora sojae isolates from Argentina

     Fitopatología, Facultad de Agronomía, Universidad de Buenos Aires, Avenida San Martín 4453, 1417 Buenos Aires, Argentina

Araceli Marcela Ramos
Diana Dokmetzian
Silvia Edith Lopez

     Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, PROPLAME-PRHIDEB-CONICET, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina

Phytophthora sojae causes root and stem rot, one of the most important diseases of soybean worldwide. Genetic diversity of 32 Phytophthora sojae isolates of different geographic origin from Argentina was evaluated with RAPD markers. The isolates were collected from diseased soybean plants and soil samples from Santa Fe, Buenos Aires, Córdoba and Entre Ríos provinces, in the Pampeana Region. DNA was amplified with 20 decanucleotides primers. Seven primers amplified 49 fragments, of which 35 were polymorphic, indicating high variability. RAPD analysis detected intraspecific variability even among isolates of the same geographic origin.

Key words: Argentina, Phytophthora root rot, RAPD, soybean