Mycologia
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
DOI: 10.3852/mycologia.97.4.872
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bowman, S. M.
Right arrow Articles by Free, S. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Bowman, S. M.
Right arrow Articles by Free, S. J.
Agricola
Right arrow Articles by Bowman, S. M.
Right arrow Articles by Free, S. J.
Mycologia, 97(4), 2005, pp. 872-879.
© 2005 by The Mycological Society of America

Mannosyltransferase is required for cell wall biosynthesis, morphology and control of asexual development in Neurospora crassa


Shaun M. Bowman 1
Amy Piwowar 1
Maria Ciocca
Stephen J. Free 2

     Department of Biological Sciences, University at Buffalo, Buffalo, New York 14260

Two Neurospora mutants with a phenotype that includes a tight colonial growth pattern, an inability to form conidia and an inability to form pro-toperithecia have been isolated and characterized. The relevant mutations were mapped to the same locus on the sequenced Neurospora genome. The mutations responsible for the mutant phenotype then were identified by examining likely candidate genes from the mutant genomes at the mapped locus with PCR amplification and a sequencing assay. The results demonstrate that a map and sequence strategy is a feasible way to identify mutant genes in Neurospora. The gene responsible for the phenotype is a putative alpha-1,2-mannosyltransferase gene. The mutant cell wall has an altered composition demonstrating that the gene functions in cell wall biosynthesis. The results demonstrate that the mnt-1 gene is required for normal cell wall biosynthesis, morphology and for the regulation of asexual development.

Key words: conidiation, fungal cell wall biosynthesis, galactomannan, protein glycosylation, protoperithecia

2 Corresponding author. E-mail: free{at}buffalo.edu.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. M. Bowman, A. Piwowar, M. Al Dabbous, J. Vierula, and S. J. Free
Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa
Eukaryot. Cell, March 1, 2006; 5(3): 587 - 600.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by The Mycological Society of America.