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Mycologia, 96(6), 2004, pp. 1352-1354.
© 2004 by The Mycological Society of America

A PCR-RFLP based diagnostic technique to rapidly identify Seiridium species causing cypress canker


Paal Krokene 1

     Norwegian Forest Research Institute, N-1432 Ås, Norway

Irene Barnes
Brenda D. Wingfield
Michael J. Wingfield

     Department of Genetics, Tree Pathology Co-operative Programme (TPCP), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

Seiridium cardinale, S. cupressi and S. unicorne represent three distinct species of fungi that cause cankers on Cupressus species and the disease collectively known as cypress canker. These fungi cannot be distinguished reliably from each other using morphological characters or ribosomal DNA sequence data. Here we describe a RFLP assay based on digesting ß-tubulin amplicons with a single endonuclease, HaeIII, which easily can be used to distinguish among these three species. This RFLP assay provides an inexpensive and simple means of identifying Seiridium species, which include some of the most serious threats to trees in Cupressaceae.

Key words: ß-tubulin, cypress canker, diagnostics, HaeIII






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Copyright © 2004 by The Mycological Society of America.