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Mycologia, 96(3), 2004, pp. 452-462.
© 2004 by The Mycological Society of America

Differential morphogenesis of the extraradical mycelium of an arbuscular mycorrhizal fungus grown monoxenically on spatially heterogeneous culture media


Bert Bago 1

     Centro de Investigaciones sobre Desertificación (CSIC/ UV/GV), Camí de la Marjal s/n, 46470 Albal (Valencia), Spain

Custodia Cano
Concepción Azcón-Aguilar

     Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), calle Profesor Albareda 1, 18008 Granada, Spain

Julie Samson
Andrew P. Coughlan
Yves Piché

     Centre de Recherche en Biologie Forestière (CRBF), Pavillon Charles-Eugène-Marchand, Université Laval, Québec, G1K 7P4 Canada

A new in vitro experimental system was developed to study the morphogenesis of discrete regions of a single extraradical mycelium of the arbuscular mycorrhizal (AM) fungus Glomus intraradices, growing simultaneously in six different agar-based media. The media were (i) unamended water agar (WA), (ii) WA+PO43– (PO43–), (iii) WA+NO3– (NO3–), (iv) WA+NH4+ (NH4+), (v) WA+NH4++MES (NH4++MES) and (vi) minimal medium (M, complete nutrients). Each medium was amended with the pH indicator bromocresol purple. The extraradical mycelium of the fungus showed between-treatment differences in morphogenesis, architecture, formation of branched absorbing structures (BAS) and sporulation. Extraradical hyphae that developed in WA or PO43– compartments exhibited an economic development pattern, in which runner hyphae radially extended the external colony. Extraradical hyphal growth in the NO3– compartments was characterized by increased formation of runner hyphae, BAS and spores and an alkalinization of the medium. In the two NH4+-amended media (NH4+, NH4++MES), sporulation was suppressed and considerable morphological changes were noted. These results show the plasticity of G. intraradices that lets it efficiently exploit an heterogeneous substrate.

Key words: branched absorbing structures (BAS), Glomus intraradices, monoxenic culture, nitrogen source, pH, sporulation




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C. Cano and A. Bago
Competition and substrate colonization strategies of three polyxenically grown arbuscular mycorrhizal fungi.
Mycologia, November 1, 2005; 97(6): 1201 - 1214.
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Copyright © 2004 by The Mycological Society of America.