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Molecular biology

Variations in sequence and occurrence of SSU rDNA group I introns in Monilinia fructicola isolates

     Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), Centre for Plant Quarantine Pests, 3851 Fallowfield Road, Ottawa, Ontario, K2H 8P9 Canada

Mireille Prud’homme

     Health Canada, Food Directorate, Building No. 7, Tunney’s Pasture, P.L. 0700E1, Ottawa, Ontario, K1A 0L2 Canada

Allison J. Meldrum

     Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), Centre for Plant Quarantine Pests, 3851 Fallowfield Road, Ottawa, Ontario, K2H 8P9 Canada

Marie-Claude Tardif

     Health Canada, Food Directorate, Building No. 7, Tunney’s Pasture, P.L. 0700E1, Ottawa, Ontario, K1A 0L2 Canada

A group I intron of 418 base pairs in the Monilinia fructicola ribosomal small-subunit sequence was characterized. The absence of such an intron in M. laxa and M. fructigena led to a PCR test for M. fructicola identification based on the presence of this intron. The failure to amplify a PCR fragment for some isolates of M. fructicola recently lead to speculation that the intron might not be present always in M. fructicola. In this study, we analyzed 13 isolates of M. fructicola and found that the intron was absent in four isolates and we determined from sequence analysis that there are several nucleotide variations that allow the M. fructicola ribosomal SSU intron to be grouped into 6 polymorphic types.

Key words: 18S rRNA, brown rot, group I intron sequences