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Mycologia, 96(2), 2004, pp. 208-210.
© 2004 by The Mycological Society of America

Cell biology/ultrastructure

Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi


Dai Tsuchiya

     Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan

Hironori Koga

     Research Institute of Agricultural Resources, Ishikawa Agricultural College, Nonoichi, Ishikawa 921-8836, Japan

Masatoki Taga 1

     Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan

A new method for scanning electron microscopy (SEM) of fungal mitotic nuclei and chromosomes was established for two ascomycetes, Cochliobolus heterostrophus and Neurospora crassa. Nuclei and chromosomes discharged from germling cells by the germ-tube burst method were spread on a surface of a glass slide. The spreads were impregnated with osmium-thiocarbohydrazide for conductive staining, followed by coating with platinum, and observed by field-emission SEM. Ultrastructure of fungal chromosomes and nuclei was visualized by SEM for the first time.

Key words: Cochliobolus heterostrophus, germ-tube burst method, mitosis, Neurospora crassa




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