This Article Full Text Full Text (PDF) Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guerber, J. C. Articles by Johnston, P. R. Search for Related Content PubMed Articles by Guerber, J. C. Articles by Johnston, P. R. Agricola Articles by Guerber, J. C. Articles by Johnston, P. R.

Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility

     Department of Plant Pathology, University of Arkansas, 217 Plant Sciences, Fayetteville, Arkansas 72701

Peter R. Johnston

     Herbarium PDD, Manaaki Whenua-Landcare Research, Private Bag 92170, Auckland, New Zealand

A diverse collection of isolates identified as Colletotrichum acutatum, including a range of fruit-rot and foliar pathogens, was examined for mtDNA RFLPs and RFLPs and sequence variation of a 900-bp intron of the glutamine synthetase (GS) gene and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene. RFLPs of mtDNA, RFLPs of the 900-bp GS intron and sequence analysis of each intron identified the same seven distinct molecular groups, or clades, within C. acutatum sensu lato. Sequence analysis produced highly concordant tree topologies with definitive phylogenetic relationships within and between the clades. The clades might represent phylogenetically distinct species within C. acutatum sensu lato. Mating tests also were conducted to assess sexual compatibility with tester isolates known to outcross to form the teleomorph Glomerella acutata. Mating compatibility was identified within one clade, C, and between two phylogenetically distinct clades, C and J4. The C clade represented isolates from a wide range of hosts and geographic origins. J4 clade contained isolates from Australia or New Zealand recovered from fruit rot and pine seedlings with terminal crook disease. That isolates in two phylogenetically distinct clades were capable of mating suggests that genetic isolation occurred before reproductive isolation. No other isolates were sexually compatible with the mating testers, which also were in groups C and J4. Certain clades identified by mtDNA and intron analysis (D1, J3 and J6) appeared to represent relatively host-limited populations. Other clades (C1, F1 and J4) contained isolates from a wide range of hosts. Isolates described as C. acutatum f. sp. pineum were clearly polyphyletic.

Key words: anthracnose, genealogy, gloeosporioides, lupini, miyabeana, phylogeny

This article has been cited by other articles:

				P. Talhinhas, S. Sreenivasaprasad, J. Neves-Martins, and H. Oliveira Molecular and Phenotypic Analyses Reveal Association of Diverse Colletotrichum acutatum Groups and a Low Level of C. gloeosporioides with Olive Anthracnose Appl. Envir. Microbiol., June 1, 2005; 71(6): 2987 - 2998. [Abstract] [Full Text] [PDF]

				M. Du, C. L. Schardl, E. M. Nuckles, and L. J. Vaillancourt Using mating-type gene sequences for improved phylogenetic resolution of Collectotrichum species complexes Mycologia, May 1, 2005; 97(3): 641 - 658. [Abstract] [Full Text] [PDF]