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Phylogeny and genetic diversity of Bridgeoporus nobilissimus inferred using mitochondrial and nuclear rDNA sequences

     Walla Walla College, College Place, Washington 99324

David S. Hibbett

     Department of Biology, Clark University, 950 Main St., Worcester, Massachusetts 01610-1477

Joseph F. Ammirati, Jr.

     Department of Botany, University of Washington, Seattle, Washington 98195

Russell J. Rodriguez ¹

     U.S. Geological Survey, WFRC, 6505 N.E. 65th St., Seattle, Washington 98115, and Department of Botany, University of Washington, Seattle, Washington 98195

The genetic diversity and phylogeny of Bridgeoporus nobilissimus have been analyzed. DNA was extracted from spores collected from individual fruiting bodies representing six geographically distinct populations in Oregon and Washington. Spore samples collected contained low levels of bacteria, yeast and a filamentous fungal species. Using taxon-specific PCR primers, it was possible to discriminate among rDNA from bacteria, yeast, a filamentous associate and B. nobilissimus. Nuclear rDNA internal transcribed spacer (ITS) region sequences of B. nobilissimus were compared among individuals representing six populations and were found to have less than 2% variation. These sequences also were used to design dual and nested PCR primers for B. nobilissimus-specific amplification. Mitochondrial small-subunit rDNA sequences were used in a phylogenetic analysis that placed B. nobilissimus in the hymenochaetoid clade, where it was associated with Oxyporus and Schizopora.

Key words: Abies procera, Basidiomycete, conk, fungi, Fuzzy Sandoze, hymenochaetoid clade, molecular systematics, Noble fir, Noble polypore, old-growth forest, Oxyporus, Polyporaceae

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