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Tea Research Foundation of Kenya, P.O. Box 820, Kericho, Kenya
Ana Pérez Sierra 1
Department of Plant Pathology, Royal Horticultural Society, Wisley, Woking, Surrey GU23 6QB, United Kingdom
Aad Termorshuizen
Biological Farming Systems, Wageningen University, Marijkeweg 22, 6709 PG Wageningen, The Netherlands
Armillaria is a primary root rot pathogen of tea (Camellia sinensis) in Kenya. The main species presently described in this country are A. mellea and A. heimii. A survey covering fourteen districts of Kenya was carried out and forty-seven isolates of Armillaria collected. Cultural morphology, rhizomorph characteristics, somatic incompatibility and features of basidiomata were used to characterize the isolates, together with molecular analysis based on randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) and the intergenic spacer (IGS) regions and sequence of the IGS region. It can be concluded that two Armillaria species were present and they were different from A. mellea. The first group was morphologically similar to A. heimii but this was contradicted by the molecular data, suggesting that A. heimii could be a complex of several species. The second group was different from the first and morphological and molecular data strongly suggest that it could be a new Armillaria species.
Key words: Armillaria, IGS, ISSR, ITS, RAPD, RFLPs, phylogeny
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