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Mycologia, 94(3), 2002, pp. 450-460.
© 2002 by The Mycological Society of America

Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina


Paulo C. Ceresini

     Departamento de Biologia, Faculdade de Engenharia, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), 15385-000, Ilha Solteira, São Paulo, Brazil

H. David Shew

     Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695

Rytas J. Vilgalys

     Department of Biology, Duke University, Durham, North Carolina 27708

U. Liane Rosewich

     USDA-ARS, Cereal Disease Laboratory, University of Minnesota, Saint Paul, Minnesota 55108

Marc A. Cubeta 1

     Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.

Key words: migration, population subdivision, Thanatephorus cucumeris




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N. D. Charlton and M. A. Cubeta
Transmission of the M2 double-stranded RNA in Rhizoctonia solani anastomosis group 3 (AG-3).
Mycologia, November 1, 2007; 99(6): 859 - 867.
[Abstract] [Full Text] [PDF]


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Copyright © 2002 by The Mycological Society of America.