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Production of xyloglucanolytic enzymes by Trichoderma viride, Paecilomyces farinosus, Wardomyces inflatus, and Pleurotus ostreatus

     Departamento de Microbiología del Suelo y Sistemas Simbioticos, Estación Experimental del Zaidín, CSIC, Apd. 419, E-18008 Granada, Spain

Different conditions of culture medium, incubation time, concentration and surfactant were tested to determine xyloglucanase activity. Trichoderma viride, Paecilomyces farinosus, Wardomyces inflatus and Pleurotus ostreatus showed increased xyloglucanase activities when the fungi grown on microcrystalline cellulose as the sole carbon source. Endoxyloglucanase activity increased with the growth of the fungi and reached a peak on day 14 of incubation, practically 95% of the activity was associated with the extracellular fraction. Precipitation with ammonium sulfate was the best concentration method for detection of endoxyloglucanase activity of the fungi. Endoxyloglucanase activity of the fungi was increased by 4 fold with the use of the non-ionic surfactant Tween 20. Six and three bands of xyloglucanase activities were observed in T. viride and P. ostreatus, respectively, whereas both P. farinosus and W. inflatus presented only one xyloglucanase activity band. These results indicate the presence of several xyloglucanases in the saprophytic fungi examined.

Key words: concentration, culture medium, incubation time, saprophytic, surfactant, xyloglucanase