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An analysis of trehalose, glycerol, and mannitol accumulation during heat and salt stress in a salt marsh isolate of Aureobasidium pullulans

     American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Virginia, 20110-2209

Albert P. Torzilli ¹

     Department of Biology, George Mason University, Fairfax, Virginia, 22030-4444

Polyhydroxy compounds from Aureobasidium pullulans exposed to stress treatments of heat, salt, and simultaneous heat and salt were isolated, identified, and quantified. Results from both thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) showed that concentrations of trehalose, mannitol, and glycerol increased under stress conditions that induce osmotic- and thermotolerance in A. pullulans. The cellular concentration of trehalose increased in heat-stressed and in simultaneously heat- and salt-stressed cells but not in cells subjected to salt stress alone. Mannitol increased under all stress conditions examined, while an increase in intracellular glycerol was apparent only in salt-stressed cells. The significance of these findings in relation to stress tolerance in salt marsh environments is discussed.

Key words: Aureobasidium, glycerol, HPLC, mannitol, stress response, TLC, trehalose