Mycologia
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
DOI: 10.3852/08-198
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
08-198v1
102/1/54    most recent
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Permissions
Google Scholar
Right arrow Articles by Ash, G. J.
Right arrow Articles by Harper, J. D.I.
PubMed
Right arrow Articles by Ash, G. J.
Right arrow Articles by Harper, J. D.I.
Agricola
Right arrow Articles by Ash, G. J.
Right arrow Articles by Harper, J. D.I.
Mycologia, 102(1), 2010, pp. 54-61.
© 2010 by The Mycological Society of America

Genetic characterization of a novel Phomopsis sp., a putative biocontrol agent for Carthamus lanatus


Gavin J. Ash 1
Benjamin Stodart
Suchirat Sakuanrungsirikul 2
Emma Anschaw 3
Nigel Crump 4

     E.H. Graham Centre (Charles Sturt University and Industry & Investment NSW), PO Box 588, Wagga Wagga NSW 2650 Australia

Deborah Hailstones

     Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, PMB 8 Camden NSW 2570 Australia

John D.I. Harper

     E.H. Graham Centre (Charles Sturt University and Industry & Investment NSW), PO Box 588, Wagga Wagga NSW 2650 Australia

A large number of isolates of Phomopsis sp. have been collected from the weed Carthamus lanatus (saffron thistle) in Australia, and their potential as biological control agents for weeds of the Asteraceae has been demonstrated. An analysis of their genetic diversity and a multigene phylogenetic analysis were undertaken to ascertain whether these isolates were distinct from other species of Phomopsis that commonly attack crop species in Australia. Minimal variation was found between the Phomopsis spp. isolated from saffron thistle, except two isolates that appeared to share identity with Diaporthe helianthii and P. viticola. Analysis of the selected isolates from saffron thistle with the nucleotide sequence of the partial ITS and tef1-{alpha} regions demonstrated that the sequences were distinct from all other species of Phomopsis so far described from crops in Australia. These findings provide strong support for the recognition of these isolates as a separate species of Phomopsis. The implications of these findings are discussed in relation to biological control of saffron thistle.

Key words: biological control, Diaporthe, saffron thistle

1 Corresponding author. E-mail: gash{at}csu.edu.au; Phone: +61 2 69332765; Fax: +61 2 69337812






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2010 by The Mycological Society of America.