Mycologia
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DOI: 10.3852/07-074R1
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Mycologia, 100(4), 2008, pp. 590-602.
© 2008 by The Mycological Society of America

Phylogenetic relationships of Phytophthora andina, a new species from the highlands of Ecuador that is closely related to the Irish potato famine pathogen Phytophthora infestans


Luis Gómez-Alpizar

     Agronomic Research Center, Box 2060, University of Costa Rica, San Pedro, Montes de Oca, Costa Rica

Chia-Hui Hu

     Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695

Ricardo Oliva

     Centro Internacional de la Papa (CIP) PO Box 17-21, Quito, Ecuador

Gregory Forbes

     Centro Internacional de la Papa (CIP) Apartado 1558, Lima 12, Peru

Jean Beagle Ristaino 1

     Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695

Phylogenetic relationships of Phytophthora infestans sensu lato in the Andean highlands of South America were examined. Three clonal lineages (US-1, EC-1, EC-3) and one heterogeneous lineage (EC-2) were found in association with different host species in genus Solanum. The EC-2 lineage includes two mitochondrial (mtDNA) haplotypes, Ia and Ic. Isolates of P. infestans sensu lato EC-2 fit the morphological description of P. infestans but are different from any genotypes of P. infestans described to date. All isolates of P. infestans sensu lato from Ecuador were amplified by a P. infestans specific primer (PINF), and restriction fragment length patterns were identical in isolates amplified with ITS primers 4 and 5. The EC-1 clonal lineage of P. infestans sensu lato from S. andreanum, S. columbianum, S. paucijugum, S. phureja, S. regularifolium, S. tuberosum and S. tuquerense was confirmed to be P. infestans based on sequences of the cytochrome oxidase I (cox I) gene and intron 1 of ras gene. The EC-2 isolates with the Ic haplotype formed a distinct branch in the same clade with P. infestans and P. mirabilis, P. phaseoli and P. ipomoeae for both cox I and ras intron 1 phylogenies and were identified as the newly described species P. andina. Ras intron 1 sequence data suggests that P. andina might have arisen via hybridization between P. infestans and P. mirabilis.

Key words: Oomycetes, Phytophthora, potato, Stramenopiles

1 Corresponding author. E-mail: Jean_Ristaino{at}ncsu.edu. Phone: (919) 515-3257; Fax (919) 515-7716.






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Copyright © 2008 by The Mycological Society of America.