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Stress response of Nidula niveo-tomentosa to UV-A light

     Zentrum Angewandte Chemie, Institut für, Lebensmittelchemie der Leibniz Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany

Manfred Nimtz

     Helmholtz-Zentrum für Infektionsforschung, Abteilung, Biophysikalische Analytik, Inhoffenstr. 7, D-38124, Braunschweig, Germany

Ralf G. Berger

     Zentrum Angewandte Chemie, Institut für, Lebensmittelchemie der Leibniz Universität Hannover, Wunstorfer Straße 14, D-30453 Hannover, Germany

Holger Zorn ¹

     AG Technische Biochemie, Universität Dortmund, Emil-Figge-Straße 68, D-44221 Dortmund, Germany

Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus’ response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.

Key words: Basidiomycete, cDNA, de novo sequencing, 2-D electrophoresis