Mycologia
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DOI: 10.3852/mycologia.100.2.171
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Mycologia, 100(2), 2008, pp. 171-180.
© 2008 by The Mycological Society of America

Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest, USA, by internal transcribed spacer sequences and phylogenetic analysis


Jill E. Petrisko 1

     Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209

Christopher A. Pearl

     USGS Forest and Rangeland Ecosystem Science Center, 3200 SW Jefferson Way, Corvallis, Oregon 97331

David S. Pilliod 2

     Aldo Leopold Wilderness Research Institute, USDA Forest Service Rocky Mountain Research Station, Missoula, Montana 59801

Peter P. Sheridan
Charles F. Williams
Charles R. Peterson

     Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209

R. Bruce Bury

     USGS Forest and Rangeland Ecosystem Science Center, 3200 SW Jefferson Way, Corvallis Oregon 97331

We assessed the diversity and phylogeny of Saprolegniaceae on amphibian eggs from the Pacific Northwest, with particular focus on Saprolegnia ferax, a species implicated in high egg mortality. We identified isolates from eggs of six amphibians with the internal transcribed spacer (ITS) and 5.8S gene regions and BLAST of the GenBank database. We identified 68 sequences as Saprolegniaceae and 43 sequences as true fungi from at least nine genera. Our phylogenetic analysis of the Saprolegniaceae included isolates within the genera Saprolegnia, Achlya and Leptolegnia. Our phylogeny grouped S. semihypogyna with Achlya rather than with the Saprolegnia reference sequences. We found only one isolate that grouped closely with S. ferax, and this came from a hatchery-raised salmon (Idaho) that we sampled opportunistically. We had representatives of 7–12 species and three genera of Saprolegniaceae on our amphibian eggs. Further work on the ecological roles of different species of Saprolegniaceae is needed to clarify their potential importance in amphibian egg mortality and potential links to population declines.

Key words: Achlya, amphibian decline, egg, lake, Leptolegnia, oomycete, Saprolegnia ferax, S. semihypogyna

1 Corresponding author. E-mail: petrisko{at}uidaho.edu Phone: (208) 529-8376. Current address: University of Idaho, 1776 Science Center Drive, Suite 205, Idaho Falls, ID 83402.






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Copyright © 2008 by The Mycological Society of America.