This Article Full Text Full Text (PDF) Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Abad, Z. G. Articles by Louws, F. J. Search for Related Content PubMed Articles by Abad, Z. G. Articles by Louws, F. J. Agricola Articles by Abad, Z. G. Articles by Louws, F. J.

Phytophthora bisheria sp. nov., a new species identified in isolates from the Rosaceous raspberry, rose and strawberry in three continents

     United States Department of Agriculture, USDA-APHIS-PPQ-PHP-PSPI, National Identification Service (NIS), Molecular Diagnostics Laboratory (MDL), Bldg. 580, BARC-E, Powder Mill Road, Beltsville, Maryland 20705

Jorge A. Abad

     United States Department of Agriculture, USDA-APHIS-PPQ-PHP-PSPI, Plant Germplasm Quarantine Program (PGQP), Bldg. 580, BARC-E, Powder Mill Road, Beltsville, Maryland 20705

Michael D. Coffey

     World Phytophthora Collection, Department of Plant Pathology, University of California, Riverside, California 92521

Peter V. Oudemans

     P.E. Marucci Center for Blueberry Cranberry Research and Extension, Rutgers State University, Chatsworth, New Jersey 08019

Willem A. Man in ’t Veld
Hans de Gruyter

     Plant Protection Service, Department of Mycology, P.O. Box 9102, 6700 HC, Wageningen, the Netherlands

James Cunnington

     Department of Primary Industries-Knoxfield, Private Bag 15, Ferntree Gully Delivery Centre, Victoria, 3156, Australia

Frank J. Louws

     Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695

A homothallic semipapillate slow growing Phytophthora species associated with root rot of strawberries from greenhouse-grown plants in North Carolina, USA, root rot of roses in the Netherlands, and root rot of raspberry in Knoxfield, Australia, was identified. The main character of this organism is the production of paragynous antheridia with broad attachment to the oogonial wall. The morphology of the pathogen does not match that of any of the more than 85 described Phytophthora species. Phylogenetic analysis based on sequences of the internal transcribed spacer rDNA region (ITS1-5.8S-ITS2) of this taxon and those from other Phytophthora species from GenBank supports the conclusion that this organism is an unreported new species. In the phylogenetic tree with other reported Phytophthora species at the GenBank, the new species is more closely related to others in ITS clade 2 comprising semipapillate taxa including P. botryosa, P. citrophthora, P. colocasiae, P. meadii, P. citricola, P. inflata, P.tropicalis, P. capsici, Phytophthora sp. ‘glovera’ and P. multivesiculata. The most closely related species is P. multivesiculata isolated from Cymbidium orchid in the Netherlands. In this paper we describe the morphological characteristics and the phylogenetic relationships that support the description of this taxon as a new species Phytophthora bisheria sp. nov.

Key words: ITS, morphology, Oomycetes, pathogenicity, phylogenetics, Phytophthora, Straminipiles, taxonomy