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Molecular and morphological description of two new species of Stemphylium from China and France

     ¹ Department of Plant Pathology, Shandong Agricultural University, Taian, Shandong
² Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, China, Taian, Shandong
³ Department of Plant Pathology, Shandong Agricultural University, Taian, Shandong, 271018, China

Two new species of Stemphylium (anamorphic Pleospora) are described on the basis of morphological characters and molecular phylogenetic analyses. Stemphylium phaseolina and S. variabilis were isolated from diseased leaves of Phaseolus vulgaris L. in Hebei Province of China and from diseased leaves of Alliµm sativum L. in Angres, France, respectively. The two species exhibit characteristic Stemphylium morphology but are distinct from similar species based on the morphology and development of conidia. The Internal Transcribed Spacer (ITS) nuclear rDNA region and glyceraldehyde-3-phosphate dehydrogenase (gpd) genes, were sequenced. The results of Maximum-Parsimony (MP) and Maximum-Likelihood (ML) phylogenetic analyses of the combined DNA sequences of these two gene regions supported S. phaseolina and S. variabilis as two distinct phylogenetic species. The taxonomic descriptions of the two new species and their comparison with related species are presented, together with the phylogenetic analysis based on combined DNA sequences of ITS and gpd gene regions.

Key words: anamorphic fungi, gpd, ITS, phylogeny, taxonomy