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First published on February 1, 2010
Mycologia 2010
DOI: 10.3852/09-044
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© 2010 by The Mycological Society of America

Relationships among North American and Japanese Laetiporus isolates inferred from molecular phylogenetics and single-spore incompatibility reactions


Mark T Banik 1,*
Daniel L. Lindner 2
Yuko Ota 3
Tsutomu Hattori 4

     1 Center for Forest Mycology Research, US Forest Service, Madison, WI, 53705, United States of America
2 Center for Forest Mycology Research, US Forest Service, One Grifford Pinchot Drive, Madison, Wisconsin 53705-2398, USA, Madison, Wisconsin, 53726, United States of America
3 Forest Microbial devision, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan
4 Microbial Ecology Lab., Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan

Relationships were investigated among North American and Japanese isolates of Laetiporus using phylogenetic analysis of ITS sequences and single spore isolate incompatibility. Single spore isolate pairings revealed no significant compatibility between North American and Japanese isolates. ITS analysis revealed 12 clades within the core Laetiporus clade, seven of which are known to occur in North America (including Hawaii and the Caribbean), three in Japan, two in South America, three in Europe and one in South Africa. The identity of L. sulphureus s.s. has yet to be determined, and could be either L. "sulphureus" (clade C), which appears to be restricted to Europe and occurs on angiosperms and gymnospersm, or L. "sulphureus" (clade E), which is found in Europe, North America and South America exclusively on angiosperms. Three clades, one from the Caribbean, one from Hawaii, and one from South Africa, have yet to be formally named. Of the three Laetiporus species found in Japan, two have been recently named (L. cremeiporus and L. montanus) and one has been epitypified (L. versisporus). The single spore incompatibility and ITS data support recognition of the three Japanese taxa as distinct biological and evolutionary species.

Key words: evolution, Fungi, Polyporaceae, Polyporales, root rot


* Center for Forest Mycology Research, US Forest Service, Madison, WI, 53705, United States of America mbanik{at}fs.fed.us







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Copyright © 2010 by The Mycological Society of America.